Data from the canadian national gonococcal antimicrobial susceptibility comparison program, including results from 25 proficiency panels distributed between 2003 and 2012, were analyzed. Ampicillin, coamoxiclav, cefuroxime and cefaclor minimum inhibitory concentrations mics were determined by etest on haemophilus test medium. The clinical and laboratory standards institute clsi subcommittee on antimicrobial. Mycobacterium tuberculosis drug susceptibility testing. Susceptibility testing of fastidious bacteria 21 6. The clinical and laboratory standards institute clsi subcommittee on antimicrobial susceptibility testing has recognized that many published studies reporting the performance of commercial asts casts suffer from major design andor analysis flaws, rendering the results.
The clinical importance of antimicrobial susceptibility test results requires that these tests be. Twelfth informational supplement mic interpretative standards as. Twentysecond informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute. This document includes updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02, m07, and m11. Educational susceptibility testing as a critical component.
Antifungal susceptibility testing, ul standards pdf, hei standards, engine performance pdf, performance metrics pdf, performanceflatplatevsvacuumtube. Educational susceptibility testing as a critical component of. Apr 11, 2016 the clinical laboratory standards institute clsi and the european committee on antimicrobial susceptibility testing eucast guidelines are the most popular breakpoint guidelines used in antimicrobial susceptibility testing worldwide. The staphylococcus aureus were tested for methicillin susceptibility using 5. Antimicrobial susceptibility of invasive haemophilus influenzae, 2012 the antimicrobial susceptibility of all 60 viable invasive isolates of h. Performance standards for antimicrobial disk susceptibility tests, th edition view sample pages this standard covers the current recommended methods for disk susceptibility testing and criteria for quality control testing. The accuracy of the phoenix automated system in combination with 2012 eucast breakpoints against recent clinical isolates was evaluated. National committee for clinical laboratory standards corporate author. Quality assurance for antimicrobial susceptibility testing. Carbapenem resistances in enterobacteriaceae, can commercial. Performance standards for antimicrobial disk and dilution. Twentythird informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute.
In this document, we attempt to answer many of the frequently asked questions regarding antimicrobial susceptibility testing. Twentyfourth informational supplement abstract the supplemental information presented in this document is. Setting male and female std clinic of regional std. The clsi publication, performance standards for antimicrobial disk susceptibility tests. Quality assurance for antimicrobial susceptibility testing of neisseria gonorrhoeae in canada, 2003 to 2012 pam sawatzky, a gary liu, a joanne r. Purpose the purpose of the kirbybauer disk diffusion susceptibility test is to. Silley 2012 has argued that the terms resistant and sensitive should. Direct antimicrobial susceptibility testing of positive. Clinical and laboratory standards institute document m02a11performance standards for antimicrobial disk susceptibility tests. Hakemivala associate prof of sbmu in the name of god panel of. Performance standards for antimicrobial susceptibility testing clsi.
Antimicrobial susceptibility testing for many years, and she is a member of two clsi working. M100 performance standards for antimicrobial susceptibility testing, 30th edition. Performance standards for antimicrobial susceptibility testing, twentyninth edition, m100ed29e. Areas of technical uncertainties atu and information on how to handle unavoidable technical uncertainty in antimicrobial susceptibility testing. Twentysecond informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute clsiapproved standards. Approved standardeleventh edition includes a series of. European committee on antimicrobial susceptibility testing 2012. Performance of standards for antimicrobial susceptibility testing ast clsi 2016 presented by. M02a11, performance standards for antimicrobial disk susceptibility tests. Atu and information on how to handle unavoidable technical uncertainty in. United states department of health and human services.
Performance standards for antimicrobial susceptibility testing. The subcommittee has worked diligently to improve m31a2performance standards for antimicrobial disk and dilution. Antimicrobial sensitivity tests, control and prevention. Eucast breakpoint criteria are being adopted by automatic antimicrobial susceptibility testing systems. Dillon, b vanessa allen, c brigitte lefebvre, d linda hoang, e greg tyrrell, f paul van caeseele, g paul levett, h and irene martin a. Of particular importance is the concept of efficacy ratios. The performance of antimicrobial susceptibility testing programmes.
Laboratory standards institute antimicrobial susceptibility testing. The colony counts of the inoculum were performed on drugfree agar medium and. Interpretation is made in accordance to the guidelines laid down in the nccls m100s12. Kirbybauer disk diffusion susceptibility test protocol. Twentieth informational supplement on free shipping on qualified orders. These tests can be useful for screening isolates to categorize them as susceptible, intermediate, resistant, or non. Susceptibility tests for bacteria isolated from animals. Performance standards for antimicrobial disk susceptibility tests, th edition view sample pages this standard covers the current recommended methods for disk susceptibility testing and.
Laboratory standards institute antimicrobial susceptibility testing standards m02 a12. Buy performance standards for antimicrobial susceptibility testing. Laboratory methodologies for bacterial antimicrobial susceptibility testing 4 oie terrestrial manual 2012 i disk diffusion, ii broth dilution, iii agar dilution. Use of trade names and commercial sources is for identification only and does not imply. Quality control in antimicrobial susceptibility testing 29 8. Standards institute antimicrobial susceptibility testing standards. See also the eucast aminoglycoside guidance document for the use of the revised aminoglycoside breakpoints. Appropriately performed qc procedures monitor a test systems precision i. Standard methods for the detection of antimicrobial resistance. Clsi m07ed11 and m100ed29 package methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, eleventh edition, m07ed11e. Testing for these drugs is done using the kirby bauer method at this time, since they are not yet available for testing using the broth microdilution method.
Twentyfifth informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute. Performance standards for antimicrobial disk susceptibility tests. Approved standard third edition describes the standard agar disk diffusion method, as well as standard. Determination of disk diffusion and mic quality control guidelines. Antimicrobial susceptibility testing of bacteria isolated from aquatic animals and their environments. Munro, cls, mt ascp ast qc is a critical component of a quality control plan qcp to ensure reliable patient results. Performance standards for antimicrobial susceptibility testing 2001 ferraro, mary jane. American proficiency institute 2012 1st test event antimicrobial susceptibility testing ungraded participants were asked to perform antimicrobial susceptibility testing on this k. M02ed performance standards for antimicrobial disk. In june 2010, the clinical and laboratory standards institute clsi published new mic and disk diffusion interpretive criteria i.
However, investigators must remain cognizant that the performance characteristics of. Comparative assessment of cds, clsi disc diffusion and etest. June 2010, january 2011, january 2012, january 20, january 2014, january 2015. Errors in interpretation and reporting results 28 7. Clinical and laboratory standards institute, wayne, pa. These changes apply to penicillins, cephalosporins and monobactams, and are. Approved standard 9th edition, represents the standard for clinical laboratories performing susceptibility testing today. The clinical laboratory standards institute clsi and the european committee on antimicrobial susceptibility testing eucast guidelines are the most popular breakpoint guidelines used in antimicrobial susceptibility testing worldwide. The clinical and laboratory standards institute clsi and the european committee on antimicrobial susceptibility testing eucast recently changed their recommendations concerning the interpretation and reporting of in vitro drug susceptibility testing dst results. This document provides updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02a11, m07a9, and m11. Quality assurance for antimicrobial susceptibility testing of. Subcommittee on antimicrobial susceptibility testing since 2012. Assessment of the phoenix automated system and eucast.
The average mic agreement between the participating laboratories ranged from 85. Standards for testing the in vitro antimicrobial susceptibility of bacteria that grow anaerobically are found in clsi document m11. Effective evaluations of antimicrobial susceptibility tests asts require robust study design. Clinical breakpoints fungi candida and aspergillus guidance for organisms not listed in breakpoint tables. Objective the aim of this study was to find a costeffective, reliable and easily applicable microbiological method to detect antimicrobial susceptibilities of n. Instituterecommended methods for disk susceptibility testing, criteria for. The importance of quality control qc for antimicrobial. Performance standards for antimicrobial susceptibility testing this document includes updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02, m07, and m11. M100s performance standards for antimicrobial susceptibility testing this document provides updated tables for the clinical and laboratory standards institute. This document provides updated tables for the clinical and laboratory standards institute antimicrobial susceptibility testing standards m02a11, m07a9, and m11a8. Comparison of clinical laboratory standards institute and. Application of computers in antimicrobial susceptibility testing 39 10. There are two internationally recognized standard methods for the performance of antifungal susceptibility testing of candida spp.
Laboratory standards institute antimicrobial susceptibility testing standards m02a12. Results are reported as the mic in micrograms or unitsml. A clinical and laboratory standards institute clsi m23style quality control qc. Performance standards for antimicrobial susceptibility testing, 24th informational supplement 24th edition. Performance standards for antimicrobial susceptibility. Comparative assessment of cds, clsi disc diffusion and. Quality assurance for antimicrobial susceptibility testing of neisseria gonorrhoeae in canada. Susceptibility testing approved these mic qc ranges in january 2012 and disk. Twentysecond informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory. International standards organisation susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices. Progress in antifungal susceptibility testing of candida. Background a variety of techniques are available for antimicrobial susceptibility testing of neisseria gonorrhoeae. Twentyfirst informational supplement abstract the supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following clinical and laboratory standards institute. Mycobacterium tuberculosis drug susceptibility testing program.